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lentiviral vector plasmid plcko  (Addgene inc)


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    Addgene inc lentiviral vector plasmid plcko
    Lentiviral Vector Plasmid Plcko, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lentiviral+plcko+vector/pLCKO+(Plasmid+%2373311)/bio_rxiv__2025__10__02__680088-60-16-20
    Average 93 stars, based on 33 article reviews
    lentiviral vector plasmid plcko - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Infection:

    Article Title: G6PD and ACSL3 are synthetic lethal partners of NF2 in Schwann cells
    Article Snippet: .. A second round of infection was performed in the surviving pooled population, with lentiviruses harboring sgRNA oligo targeting either the control AAVS1 locus or NF2 (exon2) into the lentiviral PLCKO vector (#73311 Addgene). ..

    Control:

    Article Title: G6PD and ACSL3 are synthetic lethal partners of NF2 in Schwann cells
    Article Snippet: .. A second round of infection was performed in the surviving pooled population, with lentiviruses harboring sgRNA oligo targeting either the control AAVS1 locus or NF2 (exon2) into the lentiviral PLCKO vector (#73311 Addgene). ..

    Plasmid Preparation:

    Article Title: G6PD and ACSL3 are synthetic lethal partners of NF2 in Schwann cells
    Article Snippet: .. A second round of infection was performed in the surviving pooled population, with lentiviruses harboring sgRNA oligo targeting either the control AAVS1 locus or NF2 (exon2) into the lentiviral PLCKO vector (#73311 Addgene). ..

    Article Title: G6PD and ACSL3 are synthetic lethal partners of NF2 in Schwann cells
    Article Snippet: .. Table ) were cloned into the lentiviral PLCKO vector (#73311 Addgene) according to the established protocol . .. For the inducible knockdown system, shRNAs sequences targeting G6PD, ACSL3 and control RLuc (oligos provided in Suppl.

    Clone Assay:

    Article Title: G6PD and ACSL3 are synthetic lethal partners of NF2 in Schwann cells
    Article Snippet: .. Table ) were cloned into the lentiviral PLCKO vector (#73311 Addgene) according to the established protocol . .. For the inducible knockdown system, shRNAs sequences targeting G6PD, ACSL3 and control RLuc (oligos provided in Suppl.



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    lentiviral vector plasmid plcko  (Addgene inc)
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    plcko lentiviral vector  (Addgene inc)
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    Addgene inc plcko lentiviral vector
    Development and validation of DLD-1 βcatenin-DEADPOOL cells. (A) Schematic of the βcatenin-dependent iCasp9 system. (B) AP20187 (AP) dose–response curve in DLD-1 βcatenin-DEADPOOL cells. Mean ± SD, n = 3 independent experiments. (C) Schematic of genome-wide suppressor screen to identify genes required for βcatenin signaling in CRC. (D) Crystal violet stain of DLD-1 iCasp9/Cas9 line transduced with <t>lentiviral</t> vectors enabling expression of LacZ, CTNNB1 #1, or CTNNB1 #2 gRNA and treated with EtOH or 1 nM AP20187. Representative of three independent experiments.
    Plcko Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Development and validation of DLD-1 βcatenin-DEADPOOL cells. (A) Schematic of the βcatenin-dependent iCasp9 system. (B) AP20187 (AP) dose–response curve in DLD-1 βcatenin-DEADPOOL cells. Mean ± SD, n = 3 independent experiments. (C) Schematic of genome-wide suppressor screen to identify genes required for βcatenin signaling in CRC. (D) Crystal violet stain of DLD-1 iCasp9/Cas9 line transduced with lentiviral vectors enabling expression of LacZ, CTNNB1 #1, or CTNNB1 #2 gRNA and treated with EtOH or 1 nM AP20187. Representative of three independent experiments.

    Journal: The Journal of Cell Biology

    Article Title: IPO11 mediates βcatenin nuclear import in a subset of colorectal cancers

    doi: 10.1083/jcb.201903017

    Figure Lengend Snippet: Development and validation of DLD-1 βcatenin-DEADPOOL cells. (A) Schematic of the βcatenin-dependent iCasp9 system. (B) AP20187 (AP) dose–response curve in DLD-1 βcatenin-DEADPOOL cells. Mean ± SD, n = 3 independent experiments. (C) Schematic of genome-wide suppressor screen to identify genes required for βcatenin signaling in CRC. (D) Crystal violet stain of DLD-1 iCasp9/Cas9 line transduced with lentiviral vectors enabling expression of LacZ, CTNNB1 #1, or CTNNB1 #2 gRNA and treated with EtOH or 1 nM AP20187. Representative of three independent experiments.

    Article Snippet: pLCKO lentiviral vector (#73311; Addgene) was used for gRNA construction and was performed as previously described ( ).

    Techniques: Biomarker Discovery, Genome Wide, Staining, Transduction, Expressing